Protein Sequencer from Shimadzu

Product Specs
ItemProtein Sequencer
CompanyShimadzu
Price Get Pricing Get Quote for this Product
Catalog NumberPPSQ Series
QuantityEA
ApplicationsAmino acid sequence determination using HPLC and Edman degradation

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Shimadzu

7102 Riverwood Drive

Columbia, Maryland

United States

Phone: 1-800-477-1227
1-800-477-1227
1-410-381-1222

Company Profile

Website: https://www.ssi.shimadzu.com/

(1) Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protect akuBGL activityeLifeNovember 1, 2023Xiaomei Sun, Yuxin Ye, Naofumi Sakurai, Hang Wang, Koji Kato, Jian Yu, Keizo Yuasa, Akihiko Tsuji, Min Yaostained with Ponceau S solution. The band corresponding to akuBGL was excised and analyzed using a PPSQ-53A Protein sequencer (Shimadzu, Japan) at the Instrumental Analysis Service of Hokkaido University. Effects

(2) High selectivity of the hyperthermophilic subtilase propeptide domain toward inhibition of its cognate proteaseMicrobiology SpectrumOctober 17, 2023Miha Bahun, Nataša Poklar Ulrihthe bands of interest were cut out. The N-termini were sequenced using the Edman degradation method (PPSQ-53A Gradient system; Shimadzu). The phenylthiohydantoin-amino acid derivatives were identified using a Wakosil

(3) Bacterial antiviral defense pathways encode eukaryotic-like ubiquitination systemsbioRxivOctober 1, 2023Lydia R. Chambers, Qiaozhen Ye, Jiaxi Cai, Minheng Gong, Hannah E. Ledvina, Huilin Zhou, Aaron T. Whiteley, Raymond T. Suhandynata, Kevin D. Corbettwas excised, the membrane was washed with methanol and deionized water, then loaded onto a Shimadzu PPSQ-53A instrument for analysis. Five rounds of Edman degradation were performed, and the “evaluated value”

(4) Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)Transgenic ResearchOctober 1, 2023Anne B. Carlson, Carey A. Mathesius, Tim A. Gunderson, Aideen Hession, Reba Bruyere, Henry P. Mirsky, John Zhang, Mat Sandmann, Melissa N. Fallers, Rod A. Hermanexcised and combined into a single sample which was then analyzed using an N-terminal sequencer (Shimadzu PPSQ-51A; Shimadzu; Columbia, MD) where 10 Edman degradation cycles were performed. During each cycle, the N-terminal

(5) Of Seven New K+ Channel Inhibitor Peptides of Centruroides bonito, α-KTx 2.24 Has a Picomolar Affinity for Kv1.2Toxins (Basel)August 15, 2023Kashmala Shakeel, Timoteo Olamendi-Portugal, Muhammad Umair Naseem, Baltazar Becerril, Fernando Z. Zamudio, Gustavo Delgado-Prudencio, Lourival Domingos Possani, Gyorgy Panyifull primary structure of the purified peptides was obtained by Edman degradation, using a Shimadzu Protein Sequencer PPSQ-31A/33A (Columbia, MD, USA). For this purpose, two procedures were used: direct sequencing of native

(6) The structural characterization and bioactivity assessment of nonspecific lipid transfer protein 1 (nsLTP1) from caraway (Carum carvi) seedsBMC Complementary Medicine and TherapiesJuly 20, 2023Taibah Aldakhil, Saud O. Alshammari, Bushra Siraj, Bishoy El-Aarag, Shamshad Zarina, David Salehi, Aftab Ahmedthe intact protein and the purified peptides were determined by automatic Edman degradation using a PPSQ-51A/53A gas-phase protein sequencer (Shimadzu). The retention time of the PTH-amino acid residues was compared

(7) Activation mechanism and activity of globupain, a thermostable C11 protease from the Arctic Mid-Ocean Ridge hydrothermal systemFrontiers in MicrobiologyJune 19, 2023Victoria Røyseth, Brianna M. Hurysz, Anna-Karina Kaczorowska, Sebastian Dorawa, Anita-Elin Fedøy, Hasan Arsın, Mateus Sá M. Serafim, Samuel A. Myers, Olesia Werbowy, Tadeusz Kaczorowski, et al.multi-color pre-stained protein standard (GenScript). Edman sequencing was performed on a Shimadzu PPSQ-53A at the Iowa State University Protein Facility, United States. 2.7. Mass spectrometry sample preparation Following

(8) New Insights into Immunopathology Associated to Bothrops lanceolatus Snake Envenomation: Focus on PLA2 ToxinInternational Journal of Molecular SciencesJune 9, 2023Joel J. M. Gabrili, Giselle Pidde, Fabio Carlos Magnoli, Rafael Marques-Porto, Isadora Maria Villas-Boas, Carla Cristina Squaiella-Baptistão, Felipe Silva-de-França, François Burgher, Joël Blomet, Denise V. TambourgiN-Terminal Sequencing N-terminal sequencing was performed by automated Edman degradation in a PPSQ-21A Protein Sequencer (Shimadzu, Kyoto, Japan). The dried samples were dissolved in 30 µL of TFA 0.1% and spotted on a fiberglass

(9) Proteomic data and structure analysis combined reveal interplay of structural rigidity and flexibility on selectivity of cysteine cathepsinsCommunications BiologyApril 24, 2023Livija Tušar, Jure Loboda, Francis Impens, Piotr Sosnowski, Emmy Van Quickelberghe, Robert Vidmar, Hans Demol, Koen Sedeyn, Xavier Saelens, Matej Vizovišek, et al.K, L, S, and V was performed at the Department of molecular and biomedical sciences at JSI, using a PPSQ-53A Gradient System apparatus (Shimadzu, Japan) that consisted of the following modules: a PPSQ-53A protein

(10) Activation mechanism and activity of globupain, a thermostable C11 protease from the Arctic Mid-Ocean Ridge hydrothermal systembioRxivApril 10, 2023Victoria Røyseth, Brianna M Hurysz, Anna Kaczorowska, Sebastian Dorawa, Anita-Elin Fedøy, Hasan Arsin, Mateus Serafim, Olesia Werbowy, Tadeusz Kaczorowski, Runar Stokke, et al.multi-color pre-stained protein standard (GenScript). Edman sequencing was performed on a Shimadzu PPSQ-53A at the Iowa State University Protein Facility, USA. 2.7 Size-exclusion chromatography (SEC) SEC analysis

(11) Characterization and Chemical Synthesis of Cm39 (α-KTx 4.8): A Scorpion Toxin That Inhibits Voltage-Gated K+ Channel KV1.2 and Small- and Intermediate-Conductance Ca2+-Activated K+ Channels KCa2.2 and KCa3.1Toxins (Basel)January 5, 2023Muhammad Umair Naseem, Georgina Gurrola-Briones, Margarita R. Romero-Imbachi, Jesus Borrego, Edson Carcamo-Noriega, José Beltrán-Vidal, Fernando Z. Zamudio, Kashmala Shakeel, Lourival Domingos Possani, Gyorgy Panyiamino acid sequence of the peptide was revealed by automated Edman degradation using Biotech PPSQ-31A Protein Sequencer equipment (Shimadzu Scientific Instruments, Inc., Columbia, MD, USA) according to the procedure as described

(12) Tomato (Solanum lycopersicum L.) accumulation and allergenicity in response to nickel stressScientific ReportsDecember 1, 2022Enrica Roccotiello et al.about 200 mAU at 220 nm was subjected to automated direct sequencing of the N-terminal region with a Protein Sequencer PPSQ–33B (Shimadzu Corporation, Tokyo, Japan). IgE inhibition experiments with the SPHIAa assay on

(13) Host and viral proteins involved in SARS‐CoV ‐2 infection differentially bind hemeProtein ScienceNovember 1, 2022Marie‐T. Hopp, Dhruv C. Rathod, Diana Imhofaccurate synthesis, 10 cycles of N‐terminal protein sequencing through automated Edman degradation (PPSQ‐53A, Shimadzu) of the linear precursor on bead and after cleavage from the resin were performed. For that

(14) Anti-Ovarian Cancer Conotoxins Identified from Conus VenomMoleculesOctober 5, 2022Shuang Ju, Yu Zhang, Xijun Guo, Qinghui Yan, Siyi Liu, Bokai Ma, Mei Zhang, Jiaolin Bao, Sulan Luo, Ying Futhe polyvinylidene fluoride (PVDF) membrane and dried off. The PVDF membrane was transferred onto a PPSQ-53A protein sequencer (Shimadzu, Japan). Each phenylthiohydantoin (PTH)-amino acid generated from every

(15) Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycisFrontiers in Cellular and Infection MicrobiologyAugust 15, 2022Rong Wang, Qingyan Li, Fangyan Liu, Xiaoqun Dang, Quan Sun, Xiaotian Sheng, Mingyu Hu, Jialing Bao, Jie Chen, Guoqing Pan, et al.visualized with Coomassie Bright Blue R-250, and then cut off from the membrane and tested by the PPSQ Protein Sequencer (SHIMADZU). Raw data and chromatogram were analyzed by PPSQ DataProcessing/Labsolutions. Enzymatic

(16) Cm28, a scorpion toxin having a unique primary structure, inhibits KV1.2 and KV1.3 with high affinityJournal of General PhysiologyAugust 1, 2022Muhammad Umair Naseem, Edson Carcamo-Noriega, José Beltrán-Vidal, Jesus Borrego, Tibor G. Szanto, Fernando Z. Zamudio, Gustavo Delgado-Prudencio, Lourival D. Possani, Gyorgy Panyiprimary structure of pure peptide was determined by automated Edman degradation using Biotech PPSQ-31A Protein Sequencer equipment (Shimadzu Scientific Instruments, Inc.) following the same procedure as described for another

(17) A Novel Dimeric Conotoxin, FrXXA, from the Vermivorous Cone Snail Conus fergusoni, of the Eastern Pacific, Inhibits Nicotinic Acetylcholine ReceptorsToxins (Basel)July 26, 2022Ximena C. Rodriguez-Ruiz, Manuel B. Aguilar, Mónica A. Ortíz-Arellano, Helena Safavi-Hemami, Estuardo López-Veraamino acid sequence, one nmol of αD-FrXXA was analyzed by automated Edman degradation using a PPSQ-31A Protein Sequencer (Shimadzu Scientific Instruments; Tokio, Japan) by Dr. Fernando Zamudio at Biotechnology Institute,

(18) Isolation and characterization of five novel disulfide-poor conopeptides from Conus marmoreus venomJournal of Venomous Animals and Toxins including Tropical DiseasesMay 18, 2022Ying Fu, Yu Zhang, Shuang Ju, Bokai Ma, Wenwen Huang, Sulan Luoperformed with the online NCBI or Swiss-prot database. The Edman degradation sequencing was performed in a PPSQ-53A Protein sequencer (Shimadzu, Japan). Each reduced peptide was dissolved by 20 μL ddH2O. Ten microliter

(19) A Novel Angiotensin I-Converting Enzyme Inhibitory Peptide Derived From Goat Milk Casein Hydrolysate Modulates Angiotensin II-Stimulated Effects on Vascular Smooth Muscle CellsFrontiers in NutritionApril 11, 2022Zijiao Qiao et al.Daltonik GmbH, Bremen, Germany). The N-terminal sequence of the purified peptides was identified by Protein Sequencer using the Edman degradation procedure (PPSQ-53A, Shimadzu, Japan). Peptide Syntheses The peptide (FPQYLQYPY)

(20) Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2bJournal of pharmaceutical analysisApril 1, 2022Qin X, Duan M, Pei D, Lin J, Wang L, Zhou P, Yao W, Guo Y, Li X, Tao L, et al.mg. 3.4 Physicochemical properties of I22 The membranes were transferred to an N-terminal sequencer (PPSQ-53A; Shimadzu, Shanghai, China). The sequence of 16 amino acids at the N-terminal was GHHHHHHQVQLVESGG,

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